mlpB, a gene encoding a new lipoprotein in Myxococcus xanthus

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mlpB, a gene encoding a new lipoprotein in Myxococcus xanthus.

AIMS To search for and study the genes involved in the regulation of phosphate in the soil developmental bacterium Myxococcus xanthus. METHODS AND RESULTS The mlpB gene encoding a 149 residue polypeptide was identified while screening for genes with products related to phosphate metabolism. The amino terminal 19 residues of MlpB encode a typical prokaryotic signal sequence with a putative lip...

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MlpA, a lipoprotein required for normal development of Myxococcus xanthus.

The mlpA gene encoding a 236-residue polypeptide has been identified immediately downstream of the oar gene of Myxococcus xanthus (M. Martinez-Canamero, J. Munoz-Dorado, E. Farez-Vidal, M. Inouye, and S. Inouye, J. Bacteriol. 175:4756-4763, 1993). The amino-terminal 21 residues of MlpA encode a typical prokaryotic signal sequence with a putative lipoprotein cleavage site. When expressed in Esch...

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A new Myxococcus xanthus gene cluster for the biosynthesis of the antibiotic saframycin Mx1 encoding a peptide synthetase.

The gene cluster for the biosynthesis of the heterocyclic quinone antibiotic saframycin Mx1 of Myxococcus xanthus DM504/15 was inactivated and tagged by Tn5 insertions. The tagged genes were cloned in Escherichia coli and used to select overlapping cosmid clones spanning 58 kb of the M. xanthus genome. Gene disruption experiments defined a > or = 18 kb contiguous DNA region involved in saframyc...

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Lipolytic enzymes in Myxococcus xanthus.

The genome of Myxococcus xanthus encodes lipolytic enzymes in three different families: patatin lipases, alpha/beta hydrolases, and GDSL lipases. One member of each family was characterized. The protein encoded by MXAN_3852 contains motifs characteristic of patatins. MXAN_5522 encodes a protein with the G-X-S-X-G motif characteristic of the lipase subfamily of alpha/beta hydrolases. MXAN_4569 e...

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Accordion waves in Myxococcus xanthus.

Myxococcus xanthus are Gram-negative bacteria that glide on solid surfaces, periodically reversing their direction of movement. When starved, M. xanthus cells organize their movements into waves of cell density that sweep over the colony surface. These waves are unique: Although they appear to interpenetrate, they actually reflect off one another when they collide, so that each wave crest oscil...

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ژورنال

عنوان ژورنال: Journal of Applied Microbiology

سال: 2002

ISSN: 1364-5072,1365-2672

DOI: 10.1046/j.1365-2672.2002.01517.x